PRODUCT

αβ-T・γδ-T Cell culture medium and related products

EALI515-T

EALI515-T is a serum-free medium designed for culture of human and murine peripheral Lymphocytes.

  • The large scale culture of peripheral T-lymphocyte is an important technique for immunological research and clinical cancer immune-therapy. This medium supports the proliferation and activation the various lymphocytes such as αβ-T, γδ-T, CTL, NK-T and NK cells can be used for cancer immuno-therapy.
EALI515-T

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Product Description Code Package Store at
EALITM515-T
(Without IL-2)		 Originally modified medium based on Iscove’s modified Dulbecco’s Medium (IMDM).
Basal medium for low-serum or serum-free culture of human and mouse Lymphoblast cells.
pH: 7.1-7.6
Osmolarity: 280-300 mOsm/Kg H2O
Membrane filtered, Endotoxin tested, Cell culture teste		 1502 1,000 mL 2～10℃
Notice
  • ・*Culture media and reagents are not for medicinal use, laboratory use only.
  • ・Antibiotics not include.
  • ・Capacities and containers other than those listed above are also available.Please feel free to contact us.
Inquiries about products. Request of COA

NK cell culture medium

EALI515-NK

EALI515-NK is a serum-free medium originally modified based on Iscove’s modified Dulbecco’s Medium (IMDM) for culture of human Natural killer cell (NK cell) .

  • The large scale culture of peripheral NK cell is an important for immunological research and clinical cancer immune-therapy. This medium supports the activation and proliferation of the NK cells can be used for cancer immuno-therapy.
EALI515-NK

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Product Description Code Package Store at
EALITM515-NK
(Without IL-2)		 Basal medium for low-serum or serum-free culture of human and mouse Lymphoblast & NK cells.
pH: 7.1-7.6
Osmolarity: 280-300 mOsm/Kg H2O
Membrane filtered, Endotoxin tested, Cell culture tested.		 1602 1,000 mL 2～10℃
EALITM515-NK
(IL-2 700 IU/mL)		 Recommend for expansion culture of NK cells.
Containing 700 IU/mL of IL-2.
pH: 7.1-7.6
Osmolarity: 280-300 mOsm/Kg H2O
Membrane filtered, Endotoxin tested, Cell culture tested.		 1622 1,000 mL 2～10℃
EALITM515-NK
(IL-2 1,000 IU/mL)		 Recommend for early stage culture of NK cells.
Containing 1,000 IU/mL of IL-2.
pH: 7.1-7.6
Osmolarity: 280-300 mOsm/Kg H2O
Membrane filtered, Endotoxin tested, Cell culture tested		 1632 1,000 mL 2～10℃
Notice
  • ・*Culture media and reagents are not for medicinal use, laboratory use only.
  • ・Antibiotics not include.
  • ・Capacities and containers other than those listed above are also available.Please feel free to contact us.
Inquiries about products.Request of COA

Serum free/Xeno free medium for Mesenchymal Stem Cells (MSCs) and related products

αMSCM
  • αMSCM is an originally modified αMEM that support Xeno-free & Low-serum culture of Mesenchymal Stem Cells derived from Dental pulp, Umbilical cord, Bone marrow and Adipose tissue
αMSCM 1
  • αMSCM 2
  • αMSCM 3
  • αMSCM 4

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Product Description Code Package Store at Date
Basal medium
(modified αMEM)
αMSCM Basal		 A basal medium without L-Glutamine and Cell Growth Factors (GFs). (containing rh-Transferrin and rh-Insulin).
This product can be used for low serum culture of various MSCs. Add 2mM L-Glutamine, Cell growth Factors and 2% Fetus Bovine Serum (FBS) before use.
Recommend use of x100 3GF Supplement (code: 3151F) as additive.		 1801 500 mL 2～10℃
Xeno-Free culture mediumαMSCM+PL This Product is a set of Xeno-Free medium and human Platelet Lysate (hPL:UltraGro*). No need additional growth supplements, serum and cell adhesion molecule. 1811 500 mL 2～10℃
Cell Growth factor mixturex100 3GF Supplement A mixture of recombinant human FGF-2, PDGF-BB and TGF-β1. Contains recombinant human albumin as a stabilizer.
This product is lyophilized.		 3151F 5 mg 4~8°C

＊ "UltraGRO" is a trademark of "AventaCell BioMedical Corp." Atlanta, USA.

Notice
  • ・*Culture media and reagents are not for medicinal use, laboratory use only.
  • ・Antibiotics not include.
  • ・Capacities and containers other than those listed above are also available.Please feel free to contact us.
Inquiries about products.Request of COA

Chemically defined media for protein manufacture.

EALITM515-MAｂ

Hybridoma Culture and Antibody Production.

  • “EALI 515-MAb” medium is an exclusive medium for Hybridoma cell culture and antibody production in vitro. This is especially designed as animal protein free medium without serum albumin. Various hybridoma cells grow well and maintain antibody productivity in the medium. The proteins that contained in this product are Insulin and Transferrin only, therefore the use of this medium makes simplify the recover process of monoclonal antibody from the cultured supernatant compared with serum or albumin containing medium.
EALI 515-MAｂ

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Product Description Code Package Store at
EALI 515-MAb Newly designed chemically defined medium of Hybridoma cell culture and production of Monoclonal Antibody. Containing hr-Insulin and hr-Transferrin.
Without Serum Albumin or other animal derived proteins.
pH: 7.3-7.7
Osmolarity: 280-300 mOsm/Kg H2O
Membrane filtered, Endotoxin tested, Cell culture tested.		 1402 1,000 mL 2～10℃
Notice
  • ・*Culture media and reagents are not for medicinal use, laboratory use only.
  • ・Antibiotics not include.
  • ・Capacities and containers other than those listed above are also available.Please feel free to contact us.
Inquiries about products.Request of COA

Other cell culture related products

Dulbecco’s Phosphate Buffered Saline, “PBS(-)”

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Description Components Code Pkg. Size Store at

Dulbecco’s Phosphate–Buffered Saline

pH: 7.3-7.5
Osmolarity: 276-284 mOsm/Kg H2O
Membrane filtered
Endotoxin tested
NaCl 8.00 g/L
Na2HPO4 1.15
KH2PO4 0.20
KCl 0.20

Filtered by 0.1μm membrane.

 2101 500 mL Room temp.
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TactimabTM（Anti-CD3 monoclonal antibody）

Tactimab : Anti-CD3 monoclonal antibody for human T Cell culture.

  • Manufactured by cell culture procedure using serum/animal-protein free medium, EALI515-MAb medium, originally developed.
  • Purity> 95%.
  • Isotype: IgG2a.
  • The quality check finished such as a sterility test, an examination for endotoxin, the cell proliferation study.
  • Preservative non-implication.
Tactimab

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Product Description Code Package Store at
Anti CD3 MAb for CIKTactimabTM

Affinity purified anti-CD3 monoclonal antibody without preservative.
Manufactured by animal protein free culture of mouse Hybridoma clone OKT3 cell.
Stimulate the growth of human T Lymphocyte in vitro.

 
  • Membrane filtered.
  • Endotoxin tested.
  • T cell culture tested.
  • This product is lyophilized.
 3121F 1 ㎎ Freezing
3122F 10 ㎎
Notice
  • ・*Culture media and reagents are not for medicinal use, laboratory use only.
  • ・Antibiotics not include.
Inquiries about products.Request of COA

Immuno-chemical Detection Kit and Reagents

CTX-ELISA 1B

Enzyme Linked Immuno-Sorbent Assay (ELISA) system to detect CTX1B and 54-deoxyCTX1B

  • Ciguatoxins (CTXs) are the causative toxins of marine food poisoning “ciguatera.” The “CTX-ELISA 1B” is first product in the world that can detect and quantify CTX1B and 54- deoxy CTX1B, very easily, rapidly, sensitively, specifically and at a low cost.
Immuno-chemical Detection Kit and Reagents

Property

  • Easy： Include all reagents necessary for operation in the kit.
  • Rapid： Short analysis time within 2 hours.
  • Specific：Specific to CTX1B and 54-deoxy CTX1B, no reactivity to CTX3C series.
  • Low cost：No need for expensive HPLC or Mass Spectrometer.
  • Highly Sensitive： Detection range is 100~3 pg/mL (~3 ppt) by colorimetry, and 200~0.3 pg/mL (~0.3 ppt) by fluorometry.
  • CTX-ELISA 1B
  • CTX-ELISA 1B

This kit contains sufficient reagents for 2 x 96 determinations.

Code Components Quantity
8111 Plate Wash Buffer x20 conc, W-1 1 x 50 mL
8112 STD/Sample Diluent, D-1 1 x 50 mL
8113 Anti-CTX1B-ALP Diluent, D-2 1 x 50 mL
8114 ALP Substrate Solution (pNPP), R-1 1 x 50 mL
8115 ELISA Assay Plate CTX1B　 2 x 96 well
8116 Anti-CTX1B-ALP：Freeze dry powder 1 tube
8117 CTX-1B STD 5 ng/mL 1 x 100 μL
-- Adhesive plastic sheet 6 sheet
-- Operation manual 1
CTX-ELISA Related Products and reagents

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Product Description Code Pkg. Size Store at
Plate Wash Buffer
x20 conc.
W-1		 x20 concentrated Tris-buffer(pH 7.5) with tween 20, blocking reagent and preservative.
20 hold dilute with pure water before use.		 8111 50 mL 2 ～ 10˚C
STD/Sample
Diluent
D-1		 Specially developed buffer solution to dissolve hydrophobic Ciguatoxin molecule into the water.
pH 7.5
Ready to use.		 8112 50 mL 2 ～ 10˚C
Anti-CTX1B-ALP
Diluent
D-2		 Specially designed buffer solution(pH 7.5) for dilution of alkaline-phosphatase conjugated antibody, with Mg++, blocking material and detergent.
Ready to use.		 8113 50 mL 2 ～ 10˚C
ALP Substrate
Solution (pNPP)
R-1		 Alkaline-phosphatase substrate solution.
Alkaline solution of 0.01% disodium p-Nitrophenylphosphate (pNPP).
pH10,Useful for colorimetric assay of Alkaline-phosphatase.
Ready to use.		 8114 50 mL Dark and cool
2 ～ 10˚C
ELISA Assay Plate
CTX1B		 Anti-CTX1B antibody immobilized 96 well micro-plate.
Protected with blocking reagents to stabilize antibodies.
More than 8 months store is possible under dry conditions of 4 ℃ or less.		 8115 1 plate 2 ～ 10˚C

Related papers

Ciguatera and its off-shoots-Chance encounters en route to a molecular structure.
Scheuer, P. J.
Tetrahedron, 50, 3 (1994).
Structures and configurations of ciguatoxin from moray eel Gymnothorax javanicus and its likely precursor from the dinoflagellate Gambierdiscus toxicus. Murata, M.,
Legrand, A. M., Ishibashi, Y., Fukui, M., Yasumoto, T.
J. Am. Chem. Soc. 112, 4380 (1990).
Marine toxins. Yasumoto, T., Murata, M.
Chem. Rev. 93, 1897 (1993).
Structural elucidation of ciguatoxin congeners by fast-atom bombardment tandem mass spectroscopy. Yasumoto, T., Igarashi, T., Legrand, A.-M., Cruchet, P., Chinain,
M., Fujita, T., Naoki, H.
J. Am. Chem. Soc. 122, 4988 (2000).
The chemistry and biological function of natural marine toxins. Yasumoto, T.
Chem. Rec. 1, 228 (2001).
Total synthesis of ciguatoxin CTX3C. Hirama, M., Oishi, T., Uehara, H., Inoue, M., Maruyama, M., Oguri, H., Satake, M.
Science, 294, 1904 (2001).
Synthesis-based approach toward direct sandwich immunoassay for ciguatoxin CTX3C. Oguri, H., Hirama, M., Tsumuraya, T., Fujii, I., Maruyama, M., Uehara, H., Nagumo, Y.
J. Am. Chem. Soc. 125, 7608 (2003).
Total synthesis of ciguatoxin and 51-hydroxyCTX3C. Inoue, M., Miyazaki, K., Ishihara, Y., Tatami, A., Ohnuma, Y., Kawada, Y., Komano, K., Yamashita, S., Lee, N., Hirama, M.
J. Am. Chem. Soc. 128, 9352 (2006).
Production of monoclonal antibodies for sandwich immunoassay detection of Pacific ciguatoxins.
Tsumuraya, T., Fujii, I., Hirama, M.
Toxicon, 56, 797 (2010).
Ciguatera incidence and fish toxicity in Okinawa, Japan. Oshiro, N., Yogi, K., Asato, S., Sasaki, T., Tamanaha, K., Hirama, M., Yasumoto, T., Inafuku, Y.
Toxicon, 56, 656 (2010).
First toxin profile of ciguateric fish in Madeira Arquipelago (Europe). Otero, P., Perez, S., Alfonso, A., Vale, C., Rodriguez, P., Gouveia, N. N., Gouveia, N., Delgado, J., Vale, P., Hirama, M., Ishihara, Y., Molgo, J., Botana, L. M.
Anal. Chem. 82, 6032 (2010).
Detailed LC-MS/MS analysis of ciguatoxins revealing distinct regional and species characteristics in fish and causative alga from the Pacific. Yogi, K., Oshiro, N., Inafuku, Y., Hirama, M., Yasumoto, T.
Anal. Chem. 83, 8886 (2011).
Development of a monoclonal antibody against the left wing of ciguatoxin CTX1B: Thiol strategy and detection using a sandwich ELISA. Tsumuraya, T., Takeuchi, K., Yamashita, S., Fujii, I., Hirama, M.
Toxicon, 60, 348 (2012).
Preparation of anti-ciguatoxin monoclonal antibodies using synthetic haptens: Sandwich ELISA detection of ciguatoxins. Tsumuraya, T.,Fujii, I., Hirama, M.
J. ACAC. 97, 373 (2014).
Practical route to the left wing of CTX1B and total syntheses of CTX1B and 54-deoxyCTX1B. Yamashita, S., Takeuchi, K., Koyama, T., Inoue, M., Hayashi, Y., Hirama, M.
Chem. Eur. J. 21, 2621 (2015).
Highly Sensitive and Practical Fluorescent Sandwich ELISA for Ciguatoxins. Tsumuraya, T., Sato, T., Hirama, M., Fujii, I.,
Anal. Chem. 90, 7318 (2018).
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