PRODUCT

αβ-T・γδ-T Cell culture medium and related products

EALI515-T

EALI515-T is a serum-free medium designed for culture of human and murine peripheral Lymphocytes.

  • The large scale culture of peripheral T-lymphocyte is an important technique for immunological research and clinical cancer immune-therapy. This medium supports the proliferation and activation the various lymphocytes such as αβ-T, γδ-T, CTL, NK-T and NK cells can be used for cancer immuno-therapy.
EALI515-T

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Product Description Code Package Store at
EALITM515-T
(Without IL-2)
Originally modified medium based on Iscove’s modified Dulbecco’s Medium (IMDM).
Basal medium for low-serum or serum-free culture of human and mouse Lymphoblast cells.
pH: 7.2-7.6
Osmolarity: 280-300 mOsm/Kg H2O
Membrane filtered, Endotoxin tested, Cell culture teste
1502 1,000 mL 2~10℃
EALITM515-T
(IL-2 175 IU/mL)
Containing 175 IU/mL of IL-2.
pH: 7.2-7.6
Osmolarity: 280-300 mOsm/Kg H2O
Membrane filtered, Endotoxin tested, Cell culture tested
1512 1,000 mL 2~10℃
1513 1,000 mL
CB*1
EALITM515-T
(IL-2 700 IU/mL)
Containing 700 IU/mL of IL-2.
pH: 7.3-7.5
Osmolarity: 280-300 mOsm/Kg H2O
Membrane filtered, Endotoxin tested, Cell culture tested
1521 500 mL 2~10℃
1522 1,000 mL

*1 CB: Gas permeable plastic bag (Culture Bag)

Notice
  • ・*Culture media and reagents are not for medicinal use, laboratory use only.
  • ・Antibiotics not include.
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TactimabTM(Anti-CD3 monoclonal antibody)

Tactimab : Anti-CD3 monoclonal antibody for human T Cell culture.

  • Manufactured by cell culture procedure using serum/animal-protein free medium, EALI515-MAb medium, originally developed.
  • Purity> 95%.
  • Isotype: IgG2a.
  • The quality check finished such as a sterility test, an examination for endotoxin, the cell proliferation study.
  • Preservative non-implication.
Tactimab

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Product Description Code Package Store at
Anti CD3 MAb for CIKTactimabTM Affinity purified anti-CD3 monoclonal antibody without preservative.
Manufactured by animal protein free culture of Hybridoma OKT3.
Stimulate the growth of human T Lymphocyte in vitro.
Membrane filtered, Endotoxin tested, Cell culture tested.
3121 1 ㎎ before use.
-80˚C

after thawing
2~8°C
3122 10 ㎎
Notice
  • ・*Culture media and reagents are not for medicinal use, laboratory use only.
  • ・Antibiotics not include.
Inquiries about products.Request of COA

NK cell culture medium

EALI515-NK

EALI515-NK is a serum-free medium originally modified based on Iscove’s modified Dulbecco’s Medium (IMDM) for culture of human Natural killer cell (NK cell) .

  • The large scale culture of peripheral NK cell is an important for immunological research and clinical cancer immune-therapy. This medium supports the activation and proliferation of the NK cells can be used for cancer immuno-therapy.
EALI515-NK

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Product Description Code Package Store at
EALITM515-NK
(Without IL-2)
Basal medium for low-serum or serum-free culture of human and mouse Lymphoblast & NK cells.
pH: 7.2-7.6
Osmolarity: 280-300 mOsm/Kg H2O
Membrane filtered, Endotoxin tested, Cell culture tested.
1602 1,000 mL 2~10℃
EALITM515-NK
(IL-2 700 IU/mL)
Recommend for expansion culture of NK cells.
Containing 700 IU/mL of IL-2.
pH: 7.2-7.6
Osmolarity: 280-300 mOsm/Kg H2O
Membrane filtered, Endotoxin tested, Cell culture tested.
1622 1,000 mL 2~10℃
EALITM515-NK
(IL-2 1,000 IU/mL)
Recommend for early stage culture of NK cells.
Containing 1,000 IU/mL of IL-2.
pH: 7.3-7.5
Osmolarity: 280-300 mOsm/Kg H2O
Membrane filtered, Endotoxin tested, Cell culture tested
1631 500 mL 2~10℃
1632 1,000 mL
Notice
  • ・*Culture media and reagents are not for medicinal use, laboratory use only.
  • ・Antibiotics not include.
Inquiries about products.Request of COA

Serum free/Xeno free medium for Mesenchymal Stem Cells (MSCs) and related products

αMSCM

αMSCM is an originally modified αMEM that support Xeno-free & Low-serum culture of Mesenchymal Stem Cells derived from Dental pulp, Umbilical cord, Bone marrow and Adipose tissue.

  • αMSCM 1
  • αMSCM 2
  • αMSCM 3

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Product Description Code Package Store at Date
Basal medium
(modified αMEM)
αMSCM Basal
A basal medium without L-Glutamine and Cell Growth Factors (GFs). (containing rh-Transferrin and rh-Insulin).
This product can be used for low serum culture of various MSCs. Add 2mM L-Glutamine, Cell growth Factors and 2% Fetus Bovine Serum (FBS) before use.
Recommend use of 200mM L-Glutamine Solution (code: 3131) and x100 3GF Supplement (code: 3151) as additive.
1801 500 mL 2~10℃
Xeno-Free culture mediumαMSCM+PL Complete medium containing L-Glutamine and human Platelet Lysate (UltraGro*). No need additional growth supplements, serum and cell adhesion molecule. 1811 500 mL 2~10℃
1812 250 mL
Cell Growth factor mixture for MSCx100 3GF Supplement A mixture of recombinant human FGF-2, PDGF-BB and TGF-β1. Contains recombinant human albumin as a stabilizer. 3151 5 mL before use.
-80˚C

after thawing
2~8°C
for cell culture200mM L-Glutamine solun. 200 mM (29.2 g/L) L-Glutamine PBS solution.
Sterilized and filtered by 0.2μm membrane.
Endotoxin tested, Cell culture tested.
3131 100 mL 2~10℃

* "UltraGRO" is a trademark of "AventaCell BioMedical Corp." Atlanta, USA.

Notice
  • ・*Culture media and reagents are not for medicinal use, laboratory use only.
  • ・Antibiotics not include.
Inquiries about products.Request of COA

Human fibroblast cell culture medium and related products

αMFB

αMFB is an originally modified αMEM that support Xeno-free & Low-serum culture of Epidermal Fibroblast cells.

  • This medium would contribute to the safety in the field of regenerative medicine.
α-MFB

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Product Description Code Package Store at Date
αMFB basal A basal medium without L-Glutamine and Cell Growth Factors (GFs). (containing rh-Transferrin and rh-Insulin).
This product can be used for low serum culture of Fibroblast cells. Add 2mM L-Glutamine, Cell growth Factors and 2% Fetus Bovine Serum (FBS) before use. Recommend use of 200mM L-Glutamine Solution (code: 3131) and x100 3GF Supplement (code: 3151) as additive.
Membrane filtered, Endotoxin tested,
Cell culture tested.
1701 500 mL 2~10℃
1702 1,000 mL
αMFB+PL Complete medium containing L-Glutamine and human Platelet Lysate (UltraGro*). No need additional growth supplements, serum and cell adhesion molecule.
Membrane filtered, Endotoxin tested,
Cell culture tested.
1711 500 mL 2~10℃
1712 250 mL

* "UltraGRO" is a trademark of "AventaCell BioMedical Corp." Atlanta, USA.

Notice
  • ・*Culture media and reagents are not for medicinal use, laboratory use only.
  • ・Antibiotics not include.
Inquiries about products.Request of COA

Chemically defined media for protein manufacture.

EALITM515-MAb

Hybridoma Culture and Antibody Production.

  • “EALI 515-MAb” medium is an exclusive medium for Hybridoma cell culture and antibody production in vitro. This is especially designed as animal protein free medium without serum albumin. Various hybridoma cells grow well and maintain antibody productivity in the medium. The proteins that contained in this product are Insulin and Transferrin only, therefore the use of this medium makes simplify the recover process of monoclonal antibody from the cultured supernatant compared with serum or albumin containing medium.
EALI 515-MAb

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Product Description Code Package Store at
EALI 515-MAb Newly designed chemically defined medium of Hybridoma cell culture and production of Monoclonal Antibody. Containing hr-Insulin and hr-Transferrin.
Without Serum Albumin or other animal derived proteins.
pH: 7.3-7.7
Osmolarity: 280-300 mOsm/Kg H2O
Membrane filtered, Endotoxin tested, Cell culture tested.
1402 1,000 mL 2~10℃
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MaxiGroTM-CHO

Protein free synthetic medium for suspension culture of CHO cell.

  • “MaxiGro-CHO” medium is an exclusive medium for suspension culture of CHO cell. This is especially designed as chemically defined medium without any proteins and/or hydrolyzates.
  • This product does not contain L-glutamine, so can be stored for a long time. Add 4mM L-glutamine before use.
  • High-density suspension culture using a stirring culture device is possible.
5-2-3

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Product Description Code Package Store at
MaxiGro-CHO Newly designed chemically defined medium for high-density suspension culture of CHO cells.
Containing hr-Insulin and hr-Transferrin.
Without L-Glutamine
Add 2mM L-Glutamine before use. Recommend use of 200mM L-Glutamine Solution (code: 3131)
pH: 7.3-7.7
Osmolarity: 295-310 mOsm/Kg H2O
Membrane filtered, Endotoxin tested, Cell culture tested.
1412 1,000 mL 2~10℃
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Other cell culture related products

Dulbecco’s Phosphate Buffered Saline, “PBS(-)”

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Description Components Code Pkg. Size Store at

Dulbecco’s Phosphate–Buffered Saline

pH: 7.4-7.6
Osmolarity: 276-284 mOsm/Kg H2O
Membrane filtered
Endotoxin tested
Cell culture tested
NaCl 8.00 g/L
Na2HPO4 1.15
KH2PO4 0.20
KCl 0.20

Filtered by 0.1μm membrane.

2101 500 mL Room temp.
2102 1,000 mL
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HEPES Buffered Saline, “HEPES-BS with Glucose”

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Description Components Code Pkg. Size Store at

HEPES Buffered Saline with Glucose


pH: 7.4
Osmolarity: 290~300 mOsm/Kg H2O
Membrane filtered
Endotoxin tested
Cell culture tested
NaCl 8.182 g/L
KCl 0.187
CaCl2 0.200
MgCl2/6H2O 0.230
HEPES 4.760
D-Glucose 1.000

Filtered by 0.1μm membrane.

2121 500 mL Room temp.
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200mM L-Glutamine solution

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Description Components Code Pkg. Size Store at
Membrane filtered
Endotoxin tested
Cell culture tested
pH 7.2
200 mM (29.2 g/L)
L-Glutamine PBS solution
3131 100 mL -30~4℃
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Gentamicin sulfate solution

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Description Components Code Pkg. Size Store at
Antibiotics for cell culture.
Gentamicin sulfate solution.
Sterilized and filtered by 0.2μm membrane.
Cell culture tested
0.5~50 μg/mL recommend for cell culture.
10 mg/mL Gentamicin sulfate PBS solution 3141 50 mL -30~4℃
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Anti-CD3 monoclonal antibody, “Tactimab”

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Description Components Code Pkg. Size Store at

Affinity purified anti-CD3 monoclonal antibody, produced by serum-free culture of mouse hybridoma clone OKT3 cell.
Stimulate human T Lymphocyte growth in vitro.

 
  • Membrane filtered
  • Endotoxin tested
  • T cell culture tested
NaCl 8.00 mg/mL
Na2HPO4 1.15
KH2PO4 0.20
KCl 0.20
Tween80 0.2
IgG2a 1 or 10

Anti-CD3 mouse monoclonal antibody Without preservative

3121 1 mg/mL before use.
-80˚C

after thawing
2~8°C
3122 10 mg/mL
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x100 3GF Supplement

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Description Components Code Pkg. Size Store at
Cell Growth factor mixture for MSCs.
Sterilized and filtered by 0.2μm membrane.
Cell culture tested.
0.5~50μg/mL recommend for cell culture.
Mixture of recombinant human FGF-2, PDGF-BB and TGF-β1.
Contains recombinant human albumin as a stabilizer.
3151 5 mL before use.
-80˚C

after thawing
2~8°C
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Insulin solution for cellculture

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Description Components Code Pkg. Size Store at
Human recombinant Insulin acidic solution.
Sterilized and filtered by 0.2μm membrane.
Endotoxin tested, Cell culture tested
10 mg/mL acid solution.
Specific activity ≧27u/mg
3101 5 mL before use.
-80˚C

after thawing
2~8°C
Inquiries about products.Request of COA

Immuno-chemical Detection Kit and Reagents

CTX-ELISA 1B(CSI Code No. 8100, Wako Code No. 382-14341)

Enzyme Linked Immuno-Sorbent Assay (ELISA) system to detect CTX1B and 54-deoxyCTX1B

  • Ciguatoxins (CTXs) are the causative toxins of marine food poisoning “ciguatera.” The “CTX-ELISA 1B” is first product in the world that can detect and quantify CTX1B and 54- deoxy CTX1B, very easily, rapidly, sensitively, specifically and at a low cost.
Immuno-chemical Detection Kit and Reagents

Property

  • Easy: Include all reagents necessary for operation in the kit.
  • Rapid: Short analysis time within 2 hours.
  • Specific:Specific to CTX1B and 54-deoxy CTX1B, no reactivity to CTX3C series.
  • Low cost:No need for expensive HPLC or Mass Spectrometer.
  • Highly Sensitive: Detection range is 100~3 pg/mL (~3 ppt) by colorimetry, and 200~0.3 pg/mL (~0.3 ppt) by fluorometry.
  • CTX-ELISA 1B
  • CTX-ELISA 1B

This kit contains sufficient reagents for 2 x 96 determinations.

Code Components Quantity
8111 Plate Wash Buffer x20 conc, W-1 1 x 50 mL
8112 STD/Sample Diluent, D-1 1 x 50 mL
8113 Anti-CTX1B-ALP Diluent, D-2 1 x 50 mL
8114 ALP Substrate Solution (pNPP), R-1 1 x 50 mL
8115 ELISA Assay Plate CTX1B  2 x 96 well
8116 Anti-CTX1B-ALP 1 x 250 μL
8117 CTX-1B STD 5 ng/mL 1 x 100 μL
-- Adhesive plastic sheet 6 sheet
-- Operation manual 1
CTX-ELISA Related Products and reagents

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Product Description Code Pkg. Size Store at
Plate Wash Buffer
x20 conc.
W-1
x20 concentrated Tris-buffer(pH 7.5) with tween 20, blocking reagent and preservative.
20 hold dilute with pure water before use.
8111 50 mL Room temp
~ 4˚C
STD/Sample
Diluent
D-1
Specially developed buffer solution to dissolve hydrophobic Ciguatoxin molecule into the water.
pH 7.5
Ready to use.
8112 50 mL Room temp
~ 4˚C
Anti-CTX1B-ALP
Diluent
D-2
Specially designed buffer solution(pH 7.5) for dilution of alkaline-phosphatase conjugated antibody, with Mg++, blocking material and detergent.
Ready to use.
8113 50 mL Room temp
~ 4˚C
ALP Substrate
Solution (pNPP)
R-1
Alkaline-phosphatase substrate solution.
Alkaline solution of 0.01% disodium p-Nitrophenylphosphate (pNPP).
pH10,Useful for colorimetric assay of Alkaline-phosphatase.
Ready to use.
8114 50 mL Dark and cool
ELISA Assay Plate
CTX1B
Anti-CTX1B antibody immobilized 96 well micro-plate.
Protected with blocking reagents to stabilize antibodies.
More than 8 months store is possible under dry conditions of 4 ℃ or less.
8115 1 plate 4 ~ -20˚C
Manufacture
Cell Science Inc.(CSI)
82-16 Imozawa Gongenmoriyama, Aoba-ku,
Sendai, 989-3212, Japan
http://cellscience.co.jp
Sale
FUJIFILM Wako Pure Chemical Corp.
1-2 Doshomachi 3-Chome, Chuo-ku,
Osaka 540-8605, Japan
https://labchem-wako.fujifilm.com/jp/index.html
Inquiries about products.Request of COA
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